Out of 300 participants from 51 countries who vied in the 8th International Environment Sustainability Project Olympiad (INESPO) held on May 29- June 3, 2016 at Amsterdam, The Netherlands, the three contestants from the Division of Bacoor City brought home two bronze medals.
The amazing contenders, Gabriel Matthew A. De Mesa, John Christian D. Reamico, and Greyzel Joy Zoe P. Quijano, come from Bacoor National High School-Molino Main-Special Science Curriculum. They were prepared to compete in the international competition by the outstanding Science Teacher, Ms. Lilibeth M. Mayor.The principal, Dr. Anita M. Rom, and the Education Program Supervisor for Science, Dr. Helen A. Gutierrez, also joined to give moral support to the three young scientists and the teacher.
The first entry was "Inhibitory Potential of Ipomoea aquatica (Kangkong) and Basellarubra (Alugbati) Leaf Extracts on Quorum Sensing-Mediated Virulence Factor Production on Chromobacterium violaceum and Staphylococcus aureus". This study aimed to determine the inhibitory potential of Ipomoea aquatica (kangkong) and Basellarubra (alugbati) leaf extracts on quorum sensing-mediated virulence factor production on Chromobacterium violaceum and staphylococcus aureus. Plant extracts were tested for antibacterial property. The results showed that there is no zone of inhibition around the disks, thus anti-bacterial property is absent. Furthermore, the extracts were tested to Gram-positive and Gram-negative bacteria. Disk diffusion susceptibility test showed that there was no anti-bacterial property in Staphylococcus aureus. Results showed from the DNase test agar plate that there was a zone of inhibition. Extracts were slao tested on AQS assay of Chromobacterium violaceum and revealed zone of inhibition on plates. One-way ANOVA revealed that kangkong and alugbati plant extracts had significant effect on quorum sensing against Chromobacterium violaceum. From the results, this study could be used as a promising strategy to withstand antibiotic resistance and bacterial infections.
The second entry, on the other hand, was "Angiogenic Property Analysis of Kangkong (Ipomoea aquatica) Leaf Extract on the Chorioallantoic Membrane of a 14-day old Duck Embryo". This study aimed to determine the angiogenic property analysis of kangkong (Ipomoea aquatica) leaf extract on the chorioallantoic membrane (CAM) of a 14-day old duck embryo. Four treatments were prepared, each with different concentrations of kangkong extract. The extracts were placed on the CAM of duck embryos. On the 14th day of incubation, CAMs were harvested, examined and counted for blood vessels branch points. One-way ANOVA revealed that kangkong leaf extract had a significant effect on the CAM of duck embryo. The average number of collaterals decreased as the concentration of the extract increased. It was observed that the blood vessels became thinner as the concentrations became higher. This clearly showed that the leaf extract has significant effect in inhibiting the production of blood vessels. Kangkong has a potential antiangiogenic property and a possible source of chemotherapeutic drugs against tumor cells.
INESPO organizes an event that brings together so many young scientists and their supervisors from all over the world. The event promotes the cooperation and exchange of knowledge and experiences, and stimulates the emergence of young talents in various areas of science. (Galileo L. Go-Education Program Supervisor)